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In vivo interaction between CDKA and eIF4A: a possible mechanism linking translation and cell proliferation
Author(s) -
Hutchins Andrew P.,
Roberts Gethin R.,
Lloyd Clive W.,
Doonan John H.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01382-6
Subject(s) - eif4a , cyclin dependent kinase , eukaryotic translation , microbiology and biotechnology , biology , eif4e , translation (biology) , eif4g , cell cycle , cell , biochemistry , messenger rna , gene
In a proteomics‐based screen for proteins interacting with cyclin‐dependent protein kinase (CDK), we have identified a novel CDK complex containing the eukaryotic translation initiation factor, eIF4A. Reciprocal immunoprecipitations using antibodies against eIF4A indicate that the interaction is specific. The CDKA–eIF4A complex is abundant in actively proliferating and growing cells but is absent from cells that have ceased dividing. The CDKA–eIF4A complex contains kinase activity that is sensitive to the CDK‐specific inhibitor roscovitine. This interaction points to a possible molecular mechanism linking cell proliferation with translational control.