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Inhibition of angiotensin converting enzyme (ACE) activity by flavan‐3‐ols and procyanidins
Author(s) -
Actis-Goretta Lucas,
Ottaviani Javier I,
Keen Carl L,
Fraga Cesar G
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01355-3
Subject(s) - chemistry , flavan , proanthocyanidin , enzyme , angiotensin converting enzyme , biochemistry , tetramer , random hexamer , stereochemistry , polyphenol , antioxidant , biology , blood pressure , endocrinology
It was determined that flavan‐3‐ols and procyanidins have an inhibitory effect on angiotensin I converting enzyme (ACE) activity, and the effect was dependent on the number of epicatechin units forming the procyanidin. The inhibition by flavan‐3‐ols and procyanidins was competitive with the two substrates assayed: N ‐hippuryl‐ L ‐histidyl‐ L ‐leucine (HHL) and N ‐[3‐(2‐furyl)acryloyl]‐ L ‐phenylalanylglycylglycine (FAPGG). Tetramer and hexamer fractions were the more potent inhibitors, showing Ki of 5.6 and 4.7 μM, respectively. As ACE is a membrane protein, the interaction of flavanols and procyanidins with the enzyme could be related to the number of hydroxyl groups on the procyanidins, which determine their capacity to be adsorbed on the membrane surface.