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Gene expression profile linked to p53 status in hepatitis C virus‐related hepatocellular carcinoma
Author(s) -
Okada Toshimasa,
Iizuka Norio,
Yamada-Okabe Hisafumi,
Mori Naohide,
Tamesa Takao,
Takemoto Norikazu,
Tangoku Akira,
Hamada Kenji,
Nakayama Hironobu,
Miyamoto Takanobu,
Uchimura Shunji,
Hamamoto Yoshihiko,
Oka Masaaki
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01345-0
Subject(s) - hccs , hepatocellular carcinoma , gene , biology , hepatitis c virus , virology , mutant , hepatitis b virus , gene expression , microarray , cancer research , hepadnaviridae , microbiology and biotechnology , virus , genetics
To clarify the role of p53 in 22 hepatitis C virus (HCV)‐infected hepatocellular carcinomas (HCCs), we compared the gene expression profiles of HCCs with wild‐type p53 (wt‐p53) ( n =17) and those with mutant‐type p53 (mt‐p53) ( n =5) by oligonucleotide microarray analysis. Among 83 p53 ‐related genes identified by a supervised learning method, 25 were underexpressed, and 58 were overexpressed in mt‐p53 HCCs compared with wt‐p53 HCCs. With a computer search, we identified consensus p53‐binding sequences in the 3‐kb region upstream of the translation initiation site in 59 of the 83 genes, suggesting that the in vivo p53‐associated transcription system is very complicated. These data will provide additional insights into p53‐related pathogenesis in HCV‐infected HCC.