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Identification of pollen‐expressed pectin methylesterase inhibitors in Arabidopsis
Author(s) -
Wolf Sebastian,
Grsic-Rausch Slobodanka,
Rausch Thomas,
Greiner Steffen
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01344-9
Subject(s) - silique , arabidopsis , arabidopsis thaliana , pectin , pollen , biology , microbiology and biotechnology , plant cell , biochemistry , botany , chemistry , gene , mutant
Pectin methylesterases (PMEs) play an essential role during plant development by affecting the mechanical properties of the plant cell wall. Previous work indicated that plant PMEs may be subject to post‐translational regulation. Here, we report the analysis of two proteinaceous inhibitors of PME in Arabidopsis thaliana (AtPMEI1 and 2). The functional analysis of recombinant AtPMEI1 and 2 proteins revealed that both proteins are able to inhibit PME activity from flowers and siliques. Quantitative RT‐PCR analysis indicated that expression of AtPMEI1 and 2 mRNAs is tightly regulated during plant development with highest mRNA levels in flowers. Promotor::GUS fusions demonstrated that expression is mostly restricted to pollen.