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Coordinate expression of the α and β subunits of heterotrimeric G proteins involves regulation of protein degradation in CHO cells
Author(s) -
Lee Yun-Il,
Kim So-Young,
Cho Chin-Ho,
Seo MiRan,
Cho Du-Hyong,
Kwak Sahng-June,
Juhnn Yong-Sung
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01267-5
Subject(s) - heterotrimeric g protein , microbiology and biotechnology , g protein , chinese hamster ovary cell , chemistry , protein subunit , proteasome , gq alpha subunit , protein degradation , biology , biochemistry , signal transduction , receptor , gene
Individual cell types express a characteristic balance between heterotrimeric G protein α and βγ subunits, but little is known about the regulatory mechanism. We systemically examined the regulatory mechanism in CHO cells. We found that expression of Gαs, Gαi2, and Gαq proteins increased in direct proportion to the increase of Gβ1γ2 overexpressed transiently. Expression of Gβ protein also increased following overexpression of Gαs, Gαi2, and Gαq. The Gβγ overexpression stimulated degradation of Gβ in contrast to reduction of Gαs degradation. We conclude that coordinate expression of the G protein subunits involves regulation of protein degradation via proteasome in CHO cells.