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Caenorhabditis elegans dna‐2 is involved in DNA repair and is essential for germ‐line development
Author(s) -
Lee Myon Hee,
Han Sung Min,
Han Ji Won,
Kim Yun Mi,
Ahnn Joohong,
Koo Hyeon-Sook
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01243-2
Subject(s) - caenorhabditis elegans , biology , homologous recombination , germline , microbiology and biotechnology , dna , genetics , dna repair , homologous chromosome , gene
Caenorhabditis elegans germ cell proliferation and development were severely damaged in second generation dna‐2 homozygotes. Even in the first generation, a much higher incidence of aberrant chromosomes in oocytes and resultantly higher embryonic lethality were found vs. wild type, when DNA breaks were induced by γ‐rays or camptothecin. The deficiency of dna‐2 in combination with RNA interference on mre‐11 gene expression synergistically aggravated germ‐line development, especially oocyte formation. These results suggest that C. elegans Dna‐2 is involved in a DNA repair pathway paralleling homologous recombination or non‐homologous end joining with mre‐11 participation.