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A microscope‐based screening platform for large‐scale functional protein analysis in intact cells
Author(s) -
Liebel Urban,
Starkuviene Vytaute,
Erfle Holger,
Simpson Jeremy C,
Poustka Annemarie,
Wiemann Stefan,
Pepperkok Rainer
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01197-9
Subject(s) - modular design , computational biology , golgi apparatus , function (biology) , computer science , microscope , scale (ratio) , data mining , microbiology and biotechnology , biology , cell , biochemistry , programming language , pathology , medicine , physics , quantum mechanics
A modular microscope‐based screening platform, with applications in large‐scale analysis of protein function in intact cells is described. It includes automated sample preparation, image acquisition, data management and analysis, and the genome‐wide automated retrieval of bioinformatic information. The modular nature of the system ensures that it is rapidly adaptable to new biological questions or sets of proteins. Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large‐scale, cell‐based functional proteomic projects.