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Arginine kinase overexpression improves Trypanosoma cruzi survival capability
Author(s) -
Pereira Claudio A,
Alonso Guillermo D,
Ivaldi Soledad,
Silber Ariel M,
Alves Maria Júlia M,
Torres Héctor N,
Flawiá Mirtha M
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01171-2
Subject(s) - arginine kinase , arginine , adenosine triphosphate , trypanosoma cruzi , kinase , biochemistry , biology , microbiology and biotechnology , chemistry , amino acid , parasite hosting , world wide web , computer science
Arginine kinase catalyzes the reversible transphosphorylation between adenosine diphosphate (ADP) and phosphoarginine, which is involved in temporal and spatial adenosine triphosphate (ATP) buffering. Here we demonstrate that the homologous overexpression of the Trypanosoma cruzi arginine kinase improves the ability of the transfectant cells to grow and resist nutritional and pH stress conditions. The stable transfected parasites showed an increased cell density since day 10 of culture, when the carbon sources became scarce, which resulted 2.5‐fold higher than the control group on day 28. Additional stress conditions were also tested. We propose that arginine kinase is involved in the adaptation of the parasite to environmental changes.