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Spectral tuning of obelin bioluminescence by mutations of Trp92
Author(s) -
Malikova Natalia P,
Stepanyuk Galina A,
Frank Ludmila A,
Markova Svetlana V,
Vysotski Eugene S,
Lee John
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01166-9
Subject(s) - bioluminescence , photoprotein , chemistry , fluorescence , aequorin , substituent , mutant , biophysics , photochemistry , stereochemistry , biochemistry , biology , physics , intracellular , gene , quantum mechanics
The Ca 2+ ‐regulated photoprotein obelin was substituted at Trp92 by His, Lys, Glu, and Arg. All mutants fold into stable conformations and produce bimodal bioluminescence spectra with enhanced contribution from a violet emission. The W92R mutant has an almost monomodal bioluminescence ( λ max =390 nm) and monomodal fluorescence ( λ max =425 nm) of the product. Results are interpreted by an excited state proton transfer mechanism involving the substituent side group and His22 in the binding cavity.