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Direct interaction between α‐actinin and hepatitis C virus NS5B
Author(s) -
Lan Shuiyun,
Wang Hua,
Jiang Hong,
Mao Hongxia,
Liu Xiaoying,
Zhang Xiaonan,
Hu Yunwen,
Xiang Li,
Yuan Zhenghong
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01163-3
Subject(s) - ns5b , subgenomic mrna , replicon , rna , biology , microbiology and biotechnology , rna dependent rna polymerase , hepatitis c virus , virology , complementary dna , chemistry , virus , hepacivirus , dna , gene , genetics , plasmid
It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two‐hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, α‐actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B 84–95 , NS5B 466–478 , and α‐actinin 621–733 are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that α‐actinin is essential for HCV RNA replication. Our results suggest α‐actinin may be a component of the HCV replication complex.