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Motif CXCC in nitrile hydratase activator is critical for NHase biogenesis in vivo
Author(s) -
Lu Jun,
Zheng Yujuan,
Yamagishi Hiromi,
Odaka Masafumi,
Tsujimura Masanari,
Maeda Mizuo,
Endo Isao
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01070-6
Subject(s) - nitrile hydratase , biogenesis , activator (genetics) , chemistry , biochemistry , cysteine , enzyme , gene
Nitrile hydratase (NHase) activator from Rhodococcus sp. N‐771 is required for NHase functional expression. The motif 73CXCC76 in the NHase activator sequence was here revealed to be vital for its function by site‐directed mutagenesis. All three substitutions of the cysteines by serines resulted in a much lower level of expression of active NHase. Furthermore, interaction between NHase activator and NHase was detected and the critical role of NHase activator was not exhibited in the cysteine oxidization process of NHase. These findings suggest NHase activator mainly participates in iron trafficking in NHase biogenesis as an iron type metallochaperone.