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Regulation of cell adhesion signaling by synthetic glycopolymer matrix in primary cultured hepatocyte
Author(s) -
Kim Sang-Heon,
Kim Jong-Hun,
Akaike Toshihiro
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01047-0
Subject(s) - fibronectin , microbiology and biotechnology , focal adhesion , vinculin , glycopolymer , asialoglycoprotein receptor , integrin , cell adhesion , extracellular matrix , chemistry , cytoskeleton , phosphorylation , hepatocyte , biology , cell , biochemistry , in vitro , polymer , organic chemistry , copolymer
Control of cell–matrix interactions is a central principle for the design of biomaterial in tissue engineering. In this study, we evaluated a synthetic glycopolymer, which is recognized by the asialoglycoprotein receptor (ASGPR) expressed on the surface of hepatocytes, as an artificial matrix to regulate integrin‐mediated signaling. The phosphorylation of focal adhesion kinase was restricted in hepatocytes cultured on the glycopolymer compared with fibronectin. In addition, there was no reorganization of cytoskeleton‐related proteins such as actin filaments, microtubules, and vinculin in hepatocytes cultured on the glycopolymer. DNA synthesis and cyclin D1 expression were suppressed in hepatocytes grown on the glycopolymer as compared with those grown on fibronectin and collagen. The data suggest that the glycopolymer will be a good artificial matrix to regulate integrin‐mediated signaling and cell growth through the unique ASGPR–carbohydrate interaction.