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Structural requirements for nuclear localization of GCMa/Gcm‐1
Author(s) -
Hashemolhosseini Said,
Kilian Karin,
Kardash Elena,
Lischka Peter,
Stamminger Thomas,
Wegner Michael
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01037-8
Subject(s) - nls , gcm transcription factors , nuclear localization sequence , nuclear transport , subcellular localization , nuclear export signal , nuclear protein , transcription factor , bipartite graph , microbiology and biotechnology , cell nucleus , biology , computational biology , genetics , mathematics , gene , nucleus , combinatorics , cytoplasm , general circulation model , ecology , graph , climate change
GCM proteins constitute a small transcription factor family. Nuclear localization of Drosophila GCM is mediated by a typical bipartite nuclear localization sequence (NLS) close to the DNA‐binding GCM domain. Here, we have analyzed nuclear localization of the mammalian GCM proteins. Whereas GCMb/Gcm‐2 contained a classical bipartite NLS, nuclear localization of GCMa/Gcm‐1 was mediated by two regions without resemblance to known NLS, one corresponding to the amino‐terminal part of the GCM domain, the second defined as a tyrosine‐and‐proline‐rich carboxy‐terminal region. Nuclear import was counteracted by an amino‐terminal nuclear export activity. This complex regulation of subcellular localization has important implications for GCMa/Gcm‐1 function.