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Sodium channel modulating activity in a δ‐conotoxin from an Indian marine snail
Author(s) -
Sudarslal S,
Majumdar Sriparna,
Ramasamy P,
Dhawan Ritu,
Pal Prajna P,
Ramaswami Mani,
Lala Anil K,
Sikdar S.K,
Sarma Siddhartha P,
Krishnan K.S,
Balaram P
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01016-0
Subject(s) - conotoxin , sodium channel , snail , chinese hamster ovary cell , chemistry , sodium , venom , microbiology and biotechnology , biochemistry , stereochemistry , biology , biophysics , receptor , ecology , organic chemistry
A 26 residue peptide (Am 2766) with the sequence CKQAGESCDIFSQNCCVG‐TCAFICIE‐NH 2 has been isolated and purified from the venom of the molluscivorous snail, Conus amadis , collected off the southeastern coast of India. Chemical modification and mass spectrometric studies establish that Am 2766 has three disulfide bridges. C‐terminal amidation has been demonstrated by mass measurements on the C‐terminal fragments obtained by proteolysis. Sequence alignments establish that Am 2766 belongs to the δ‐conotoxin family. Am 2766 inhibits the decay of the sodium current in brain rNav1.2a voltage‐gated Na + channel, stably expressed in Chinese hamster ovary cells. Unlike δ‐conotoxins have previously been isolated from molluscivorous snails, Am 2766 inhibits inactivation of mammalian sodium channels.