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Control of mitochondrial protein import by pH
Author(s) -
Grigoriev Sergey M,
Jensen Robert E,
Kinnally Kathleen W
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)01012-3
Subject(s) - mitochondrion , inner mitochondrial membrane , translocase of the inner membrane , chemistry , yeast , biophysics , nuclear transport , mitochondrial membrane transport protein , biochemistry , microbiology and biotechnology , biology , cytoplasm , cell nucleus
Protein import into mitochondria is inhibited by protons (IC 50 pH 6.5). The channels of the import machinery were examined to further investigate this pH dependence. TOM and TIM23 are the protein translocation channels of the mitochondrial outer and inner membranes, respectively, and their single channel behaviors at various pHs were determined using patch‐clamp techniques. While not identical, increasing H + concentration decreases the open probability of both TIM23 and TOM channels. The pattern of the pH dependences of protein import and channel properties suggests TIM23 open probability can limit import of nuclear‐encoded proteins into the matrix of yeast mitochondria.