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The mouse sino‐atrial node expresses both the type 2 and type 3 Ca 2+ release channels/ryanodine receptors
Author(s) -
Masumiya Haruko,
Yamamoto Hideyuki,
Hemberger Myriam,
Tanaka Hikaru,
Shigenobu Koki,
Chen S.R.Wayne,
Furukawa Tetsushi
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00999-2
Subject(s) - ryanodine receptor , ryanodine receptor 2 , chemistry , receptor , gene isoform , intracellular , ryr1 , messenger rna , microbiology and biotechnology , medicine , endocrinology , biology , biochemistry , gene
Ca 2+ released from intracellular Ca 2+ stores is shown to be involved in pacemaker activity in the sino‐atrial (SA)‐node. However, little is known about the molecular identity of the Ca 2+ release channel/ryanodine receptor (RYR) involved in pacemaker activity. We examined the mRNA distribution of three different RYR isoforms (RYR1, RYR2, and RYR3) in the mouse SA‐node. RNase protection assay and in situ hybridization revealed that RYR2 mRNA expresses widely in the heart including the SA‐node, while RYR3 mRNA expression is limited to the SA‐node and to the right atrium. Thus, not only RYR2 but also RYR3 may participate in pacemaker activity.