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Effects of glycerol and high temperatures on structure and function of phycobilisomes in Synechocystis sp. PCC 6803
Author(s) -
Mao Hai-Bin,
Li Guo-Fu,
Li Dong-Hai,
Wu Qing-Yu,
Gong Yan-Dao,
Zhang Xiu-Fang,
Zhao Nan-Ming
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00973-6
Subject(s) - phycobilisome , synechocystis , glycerol , biophysics , mutant , chemistry , photosystem , fluorescence , dissociation (chemistry) , photosynthesis , in vivo , strain (injury) , cyanobacteria , biochemistry , photosystem ii , biology , bacteria , organic chemistry , anatomy , physics , quantum mechanics , gene , genetics , microbiology and biotechnology
The effects of glycerol and high temperatures on structure and function of phycobilisomes (PBSs) in vivo were investigated in a chlL deletion mutant of the cyanobacterium Synechocystis sp. PCC 6803. When the mutant was grown under light‐activated heterotrophic growth conditions, it contained intact and functional PBSs, but essentially no chlorophyll and photosystems. So the structural and functional changes of the mutant PBSs in vivo can be handily detected by measurement of low temperature (77 K) fluorescence emission spectra. High concentration glycerol induced an obvious disassembly of PBSs and the dissociation of phycocyanins in the rod substructures into their oligomers and monomers. PBSs also disassembled at high temperatures and allophycocyanins were more sensitive to heat stress than phycocyanins. Our results demonstrate that the chlL − mutant strain is an advantageous model for studying the mechanisms of assembly and disassembly of protein complexes in vivo.