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Boric acid reversibly inhibits the second step of pre‐mRNA splicing
Author(s) -
Shomron Noam,
Ast Gil
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00928-1
Subject(s) - rna splicing , boric acid , chemistry , messenger rna , chelation , splice , protein splicing , precursor mrna , in vitro , reagent , alternative splicing , biochemistry , biophysics , microbiology and biotechnology , biology , gene , rna , inorganic chemistry , organic chemistry
Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre‐mRNA splicing reaction causes a dose‐dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice‐site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre‐mRNA splicing.