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Differential expression of ATPAF1 and ATPAF2 genes encoding F 1 ‐ATPase assembly proteins in mouse tissues
Author(s) -
Pı́cková Andrea,
Paul Jan,
Petruzzella Vittoria,
Houštěk Josef
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00890-1
Subject(s) - messenger rna , atpase , biology , microbiology and biotechnology , gene expression , gene , adipose tissue , brown adipose tissue , skeletal muscle , biochemistry , enzyme , endocrinology
Atp11p (Atpaf1p; F 1 ‐ATPase assembly factor 1) and Atp12p (Atpaf2p; F 1 ‐ATPase assembly factor 2) are proteins required for the assembly of β (F 1 ‐β) and α (F 1 ‐α) subunits into the mitochondrial ATPase. Here we report about 100 times lower levels of ATPAF1 and ATPAF2 transcripts in relation to the mRNA levels of F 1 ‐α and F 1 ‐β in a range of mouse tissues. Quantitative reverse‐transcription polymerase chain reaction revealed nearly constant ATPAF1 expression in all tissues in both adult and 5‐day‐old mice (up to two‐fold differences), indicating that ATPAF1 rather behaves like a maintenance gene. In contrast, ATPAF2 expression differed up to 30‐fold in the tissues analysed. ATPAF2 tissue‐specific expression was also found to correlate well with mRNA levels of both F 1 ‐α and F 1 ‐β (BAT≫kidney, liver>heart, brain>skeletal muscle), showing the highest mRNA level in the thermogenic, ATPase‐poor brown adipose tissue, which is characterised by a 10‐fold decrease in ATPase/respiratory chain stoichiometry relative to the other tissues.