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A novel role for proliferin‐2 in the ex vivo expansion of hematopoietic stem cells
Author(s) -
Choong Meng Ling,
Tan Amabel C.L,
Luo Biao,
Lodish Harvey F
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00844-5
Subject(s) - haematopoiesis , ex vivo , stromal cell , stem cell , clonogenic assay , stem cell factor , hematopoietic stem cell , cell culture , bone marrow , biology , microbiology and biotechnology , in vivo , cancer research , immunology , genetics
A family of proliferin genes was discovered on a microarray analysis of hematopoiesis supportive stromal cell lines. Proliferin‐2 (PLF2) increased the frequency of long‐term culture‐initiating cells (LTC‐IC) from 1 in 340 to 1 in 256 of the primary hematopoietic stem cell (HSC)‐enriched bone marrow cells grown on MS5.1 feeder layer. A repeat using AFT024 feeder layer also showed a similar increase in LTC‐IC (from 1 in 386 cells to 1 in 260 cells). The clonogenic output of the LTC‐ICs was also increased significantly. The growth of various hematopoietic and stromal cell lines treated with PLF2 was found to increase by 4–27%, as measured by cell count and DNA synthesis assay. These findings open up the possibility of using PLF2 as a new member of the growth factor cocktails for the ex vivo expansion of HSC.