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Transposition and targeting of the prokaryotic mobile element IS 30 in zebrafish
Author(s) -
Szabó Mónika,
Müller Ferenc,
Kiss János,
Balduf Carolin,
Strähle Uwe,
Olasz Ferenc
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00814-7
Subject(s) - transposase , transposable element , zebrafish , tn10 , biology , mobile genetic elements , insertion sequence , transposition (logic) , escherichia coli , repressor , p element , dna , genetics , extrachromosomal dna , recbcd , bacteriophage mu , plasmid , gene , genome , gene expression , linguistics , philosophy
We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS 30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage λ causes transposition primarily into the vicinity of the λ operator in E. coli , and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.