Premium
Unexpected high testis‐specific transcriptional activity of the cyclin T1 promoter in transgenic mice
Author(s) -
Mata X,
Besnard N,
Le Roux K,
Tilly G,
Andréoletti O,
Hudrisier M,
Costa Da Silva J,
Laude H,
Vilotte J.L
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00806-8
Subject(s) - biology , transgene , gene , cyclin , microbiology and biotechnology , genetically modified mouse , cyclin d , transcription (linguistics) , genetics , cell cycle , linguistics , philosophy
The ubiquitously expressed cyclin T1 gene encodes for a protein involved in human immunodeficiency virus type 1 (HIV‐1) transcription activation. The goat gene was recently shown to share an expression pattern similar to that of its endogenous counterpart when incorporated into mice using a BAC insert. To assess if its promoter could target ubiquitous expression of the bovine Prnp in transgenic mice, two constructs carrying either 1 or 30 kb of cyclin T1 5′‐flanking sequences were built and microinjected. Both constructs resulted in the unexpected high male germ cell‐specific expression of the prion protein. These data re‐question the suspected location of the cyclin T1 gene regulatory elements.