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A photosystem 1 psaFJ‐null mutant of the cyanobacterium Synechocystis PCC 6803 expresses the isiAB operon under iron replete conditions
Author(s) -
Jeanjean Robert,
Zuther Ellen,
Yeremenko Nataliya,
Havaux Michel,
Matthijs Hans C.P,
Hagemann Martin
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00769-5
Subject(s) - operon , synechocystis , mutant , cyanobacteria , photosystem ii , photosystem i , chemistry , biology
A psaFJ‐null mutant of Synechocystis sp. strain PCC 6803 was characterised. As opposed to similar mutants in chloroplasts of green algae, electron transfer from plastocyanin to photosystem 1 was not affected. Instead, a restraint in full chain photosynthetic electron transfer was correlated to malfunction of photosystem 1 at its stromal side. Our hypothesis is that absence of PsaF causes oxidative stress, which triggers the induction of the ‘iron stress inducible’ operon isiAB . Products are the IsiA chlorophyll‐binding protein (CP43′) and the isiB gene product flavodoxin. Supporting evidence was obtained by similar isiAB induction in wild type cells artificially exposed to oxidative stress.