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Unidirectional effect of lauryl sulfate on the reversible NADH:ubiquinone oxidoreductase (Complex I)
Author(s) -
Grivennikova V.G,
Ushakova A.V,
Cecchini G,
Vinogradov A.D
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00765-8
Subject(s) - paracoccus denitrificans , submitochondrial particle , chemistry , ferricyanide , oxidoreductase , oligomycin , gramicidin , electron transfer , electron transport chain , nadh dehydrogenase , respiratory chain , nad+ kinase , reductase , biochemistry , photochemistry , enzyme , membrane , atpase , protein subunit , gene
Lauryl sulfate inhibits the Δ H + ‐dependent reverse electron transfer reactions catalyzed by NADH:ubiquinone oxidoreductase (Complex I) in coupled bovine heart submitochondrial particles and in vesicles derived from Paracoccus denitrificans . The inhibitor affects neither NADH oxidase (coupled or uncoupled) nor NADH:ferricyanide reductase and succinate oxidase activities at the concentrations that selectively prevent the succinate‐supported, rotenone‐sensitive NAD + or ferricyanide reduction. Possible uncoupling effects of the inhibitor are ruled out: in contrast to oligomycin and gramicidin, which increases and decreases the rate of the reverse electron transfer, respectively, in parallel with their coupling and uncoupling effects, lauryl sulfate does not affect the respiratory control ratio. A mechanistic model for the unidirectional effect of lauryl sulfate on the Complex I catalyzed oxidoreduction is proposed.