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Molecular and functional characterisation of the zebrafish ( Danio rerio ) PEPT1‐type peptide transporter 1
Author(s) -
Verri Tiziano,
Kottra Gabor,
Romano Alessandro,
Tiso Natascia,
Peric Mark,
Maffia Michele,
Boll Michael,
Argenton Francesco,
Daniel Hannelore,
Storelli Carlo
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00759-2
Subject(s) - danio , zebrafish , transporter , peptide , biochemistry , chemistry , type (biology) , biology , microbiology and biotechnology , computational biology , gene , ecology
We report the molecular and functional characterisation of a novel peptide transporter from zebrafish, orthologue to mammalian and avian PEPT1. Zebrafish PEPT1 is a low‐affinity/high‐capacity system. However, in contrast to higher vertebrate counterparts in which maximal transport activity is independent of extracellular pH, zebrafish PEPT1 maximal transport rates unexpectedly increase at alkaline extracellular pH. Zebrafish pept1 is highly expressed in the proximal intestine since day 4 post‐fertilisation, thus preceding functional maturation of the gut, first feeding and complete yolk resorption. Zebrafish PEPT1 might help to understand the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, zebrafish pept1 can be a useful marker for screening mutations that affect gut regionalisation, differentiation and morphogenesis.