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New members of the tomato ERF family show specific expression pattern and diverse DNA‐binding capacity to the GCC box element
Author(s) -
Tournier Barthélémy,
Sanchez-Ballesta Maria Theresa,
Jones Brian,
Pesquet Edouard,
Regad Farid,
Latché Alain,
Pech Jean-Claude,
Bouzayen Mondher
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00757-9
Subject(s) - dna , phylogenetic tree , genetics , biology , affinities , binding domain , binding site , nucleotide , sequence (biology) , biochemistry , gene
Four new members of the ERF (ethylene‐response factor) family of plant‐specific DNA‐binding (GCC box) factors were isolated from tomato fruit ( LeERF1–4 ). Phylogenetic analysis indicated that LeERF2 belongs to a new ERF class, characterized by a conserved N‐terminal signature sequence. Expression patterns and cis/trans binding affinities differed between the LeERFs. Combining experimental data and modeled three‐dimensional analysis, it was shown that binding affinity of the LeERFs was affected by both the variation of nucleotides surrounding the DNA cis ‐element sequence and the nature of critical amino acid residues within the ERF domain.