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Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein components
Author(s) -
Mearini Giulia,
Chichiarelli Silvia,
Zampieri Michele,
Masciarelli Silvia,
D'Erme Maria,
Ferraro Anna,
Mattia Elena
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00690-2
Subject(s) - lytic cycle , nuclear matrix , biology , viral matrix protein , origin of replication , genome , dna replication , origin recognition complex , viral replication , virus , gammaherpesvirinae , epstein–barr virus , genetics , virology , replication (statistics) , microbiology and biotechnology , herpesviridae , gene , eukaryotic dna replication , chromatin , viral disease
During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P. Within this element, we have found that a region, 580 bp long, encompassing the replicator DS element, shows the strongest affinity for the NM. In addition, by cross‐linking with cis ‐diamminedichloroplatinum, we have identified two NM proteins with an apparent molecular weight of 85 and 60 kDa that, with high affinity and specificity, bind ori P. These proteins are not induced by EBV infection, but their interaction with ori P is lost upon induction of EBV lytic cycle. These data strongly suggest that the binding of ori P to specific components of the NM is required for EBV latent replication.