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Tissue‐specific expression of two human Ca v 1.2 isoforms under the control of distinct 5′ flanking regulatory elements
Author(s) -
Pang Li,
Koren Gideon,
Wang Zhiguo,
Nattel Stanley
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00629-x
Subject(s) - gene isoform , housekeeping gene , hek 293 cells , biology , gene , microbiology and biotechnology , vascular smooth muscle , gene expression , cell type , protein subunit , regulation of gene expression , regulatory sequence , myocyte , transcriptional regulation , cell , genetics , smooth muscle , endocrinology
Transcriptional regulation may be important for L‐type Ca 2+ channel α 1C subunit (Ca v 1.2) gene expression. In this study, we found two human Ca v 1.2 isoforms, one strongly and selectively expressed in heart and the other with apparently ubiquitous expression. The promoter for the cardiac isoform has an ‘initiator’ sequence, and is active in neonatal cardiomyocytes but not in cardiac fibroblasts, H9C2 cells, human aorta‐vascular smooth muscle and HEK293 cells. The promoter for the ubiquitously expressed isoform is of the ‘housekeeping’ type and is active in all cell types examined. These data indicate specific expression patterns of two human Ca v 1.2 isoforms under the control of distinct 5′ flanking regulatory sequences.