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Adenosine‐rich elements present in the 5′‐untranslated region of PABP mRNA can selectively reduce the abundance and translation of CAT mRNAs in vivo
Author(s) -
Melo Eduardo O.,
de Melo Neto Osvaldo P.,
Martins de Sá Cezar
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00620-3
Subject(s) - messenger rna , untranslated region , three prime untranslated region , poly(a) binding protein , translation (biology) , five prime untranslated region , au rich element , microbiology and biotechnology , biology , protein biosynthesis , reporter gene , chemistry , gene expression , gene , biochemistry
The poly(A)‐binding protein (PABP) is a highly conserved eukaryotic protein whose synthesis is regulated at the post‐transcriptional level. The binding of PABP to the poly(A)‐rich element found in the 5′‐untranslated region (5′UTR) of PABP mRNA specifically inhibits its own translation. In this report, we show that similar adenosine‐rich elements in the 5′UTR of the chloramphenicol acetyl‐transferase (CAT) gene can significantly reduce the reporter mRNA abundance and translation in human 293 cells. The reduction in mRNA level, but not CAT expression, is dependent on the size of the 5′UTR poly(A) element. Furthermore, one 5′UTR‐tethered PABP molecule is enough to inhibit CAT expression without affecting its mRNA level. We propose that the control of PABP synthesis may involve mRNA decay and the repression of translation.