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Na + ‐dependent fructose transport via rNaGLT1 in rat kidney
Author(s) -
Horiba Naoshi,
Masuda Satohiro,
Ohnishi Chiharu,
Takeuchi Daisuke,
Okuda Masahiro,
Inui Ken-ichi
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00600-8
Subject(s) - fructose , brush border , transporter , transfection , chemistry , membrane , glucose transporter , biochemistry , kidney , hek 293 cells , vesicle , organic anion transporter 1 , biology , endocrinology , insulin , gene
We found a system of Na + ‐dependent uptake of fructose by rat renal brush‐border membrane vesicles. It consisted of two saturable components, and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as α‐methyl‐ D ‐glucopyranoside uptake by transfected HEK293 cells. The features of the lower affinity type of fructose transporter in the brush‐border membranes, such as affinity and substrate recognition, were very comparable with those of rNaGLT1‐transfected HEK293 cells. These results indicated that rNaGLT1 is a primary fructose transporter in rat renal brush‐border membranes.