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Identification of hepatic peroxisomal phospholipase A 2 and characterization of arachidonic acid‐containing choline glycerophospholipids in hepatic peroxisomes
Author(s) -
Yang Jingyue,
Han Xianlin,
Gross Richard W
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00581-7
Subject(s) - glycerophospholipids , peroxisome , arachidonic acid , biochemistry , chemistry , phospholipase d , identification (biology) , glycerophospholipid , choline , phospholipase , microbody , plasmalogen , biology , enzyme , phospholipid , botany , membrane , gene
Recently, a sequence encoding a novel mammalian calcium‐independent phospholipase A 2 (iPLA 2 γ) was identified in the human genome and subsequently cloned and expressed in Sf9 insect cells. Unexpectedly, expression studies in recombinant systems demonstrated the usage of multiple translation initiation codons resulting in different polypeptides. Herein, we demonstrate that hepatic iPLA 2 γ is localized to rat liver peroxisomes, possesses a molecular mass of 63 kDa and that peroxisomal membranes are highly enriched in arachidonic acid‐containing phospholipids. Collectively, these results provide the first demonstration of iPLA 2 γ in mammalian tissue and suggest the possibility that iPLA 2 γ can contribute to lipid second messenger generation by hydrolysis of peroxisomal arachidonic acid‐containing phospholipids.