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Phasing of RecA monomers on quasi‐random DNA sequences
Author(s) -
Volodin Alexander A.,
Smirnova Elena A.,
Bocharova Tatja.,
Camerini-Otero R.Daniel
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00572-6
Subject(s) - dna , monomer , phaser , sequence (biology) , nucleotide , dna sequencing , biophysics , chemistry , biology , genetics , gene , physics , polymer , organic chemistry , optics
We show that some arbitrarily chosen DNA sequences have the ability to influence the positioning of RecA monomers in RecA–DNA complexes. The preferential phase of binding of RecA monomers is shown to depend on the DNA sequence and its nucleotide composition. A simple rearrangement of bases in a limited DNA stretch influences the phasing of RecA monomers. On the other hand, that some features of DNA sequences interfere with the phasing on specific DNA sites demonstrates the existence of mechanisms for both positive and negative regulation of phasing on natural DNAs. The possible role of phasing of RecA monomers on DNA is discussed.