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A ferredoxin Arg‐Glu pair important for efficient electron transfer between ferredoxin and ferredoxin‐NADP + reductase
Author(s) -
Teshima Keizo,
Fujita Shinobu,
Hirose Syuuichi,
Nishiyama Daisuke,
Kurisu Genji,
Kusunoki Masami,
Kimata-Ariga Yoko,
Hase Toshiharu
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00559-3
Subject(s) - ferredoxin , electron transfer , ferredoxin—nadp(+) reductase , oxidoreductase , ferredoxin thioredoxin reductase , electron transport chain , reductase , chemistry , crystallography , biology , photochemistry , biochemistry , enzyme , thioredoxin reductase , glutathione
In order to elucidate the importance of a ferredoxin (Fd) Arg‐Glu pair involved in dynamic exchange from intra‐ to intermolecular salt bridges upon complex formation with ferredoxin‐NADP + oxidoreductase (FNR), Equisetum arvense FdI and FdII were investigated as normal and the pair‐lacking Fd, respectively. The FdI mutant lacking this pair was unstable and rapidly lost the [2Fe–2S] cluster. The catalytic constant ( k cat ) of the electron transfer for FdI is 5.5 times that for FdII and the introduction of this pair into FdII resulted in the increase of k cat to a level comparable to that for FdI, demonstrating directly that the Arg‐Glu pair is important for efficient electron transfer between Fd and FNR.