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The PEF family proteins sorcin and grancalcin interact in vivo and in vitro
Author(s) -
Hansen Christian,
Tarabykina Svetlana,
la Cour Jonas M,
Lollike Karsten,
Berchtold Martin W
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00518-0
Subject(s) - in vivo , in vitro , chemistry , microbiology and biotechnology , biology , biochemistry , genetics
The penta‐EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG‐2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two‐hybrid and glutathione S ‐transferase pull‐down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co‐immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta‐EF hand type.