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Structural and functional analysis of a lycopene β‐monocyclase gene isolated from a unique marine bacterium that produces myxol
Author(s) -
Teramoto Maki,
Takaichi Shinichi,
Inomata Yukie,
Ikenaga Hiroshi,
Misawa Norihiko
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00513-1
Subject(s) - lycopene , carotenoid , carotene , gene cluster , gene , biochemistry , chemistry , strain (injury) , bacteria , beta carotene , gene expression , biology , food science , genetics , anatomy
A gene coding for lycopene β‐monocyclase, which metabolizes lycopene (ψ,ψ‐carotene) to γ‐carotene (β,ψ‐carotene), was isolated for the first time from a unique marine bacterium strain P99‐3 that produces myxol (a γ‐carotene derivative). This lycopene β‐monocyclase gene (designated crtYm ) was included in the gene cluster which contained carotenoid biosynthetic gene ( crtI , crtB , crtZ , crtY , and crtA ) homologs. CrtYm, the CrtY homolog, metabolized lycopene to γ‐carotene, which was confirmed by deletion/expression analysis of the crtYm and by subsequent analysis of the metabolites from lycopene based on the retention times on high‐performance liquid chromatography, UV–visible absorption spectra, and mass spectrometry.