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Alternative splicing of type II procollagen pre‐mRNA in chondrocytes is oppositely regulated by BMP‐2 and TGF‐β1
Author(s) -
Valcourt Ulrich,
Gouttenoire Jérôme,
Aubert-Foucher Elisabeth,
Herbage Daniel,
Mallein-Gerin Frédéric
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00510-6
Subject(s) - procollagen peptidase , chemistry , type ii collagen , rna splicing , chondrocyte , cartilage , bone morphogenetic protein , microbiology and biotechnology , bone morphogenetic protein 2 , messenger rna , transforming growth factor , alternative splicing , gene expression , gene , biology , rna , biochemistry , anatomy , in vitro
Type II collagen is the major protein of cartilage and is synthesized as a procollagen in two forms (IIA and IIB), generated by differential splicing of the gene primary transcript. Previous studies have indicated that only type IIB is expressed in differentiated chondrocytes. Here, we examined the effects of bone morphogenetic protein (BMP)‐2 and transforming growth factor (TGF)‐β1 on the expression of IIA and IIB forms expressed in de‐differentiated chondrocytes grown in monolayer. Our results demonstrate that BMP‐2 favors expression of type IIB whereas TGF‐β1 potentiates expression of type IIA induced by subculture. These observations reveal the specific capability of BMP‐2 to reverse the de‐differentiation state of chondrocytes.