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In situ photoaffinity labeling of the target protein for lembehyne A, a neuronal differentiation inducer
Author(s) -
Aoki Shunji,
Matsui Kouhei,
Takata Tetsuo,
Kobayashi Motomasa
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00506-4
Subject(s) - photoaffinity labeling , neuroblastoma , chemistry , inducer , cellular differentiation , in situ , microbiology and biotechnology , biochemistry , receptor , biology , cell culture , gene , organic chemistry , genetics
A C 36 linear acetylene alcohol, lembehyne A (LB‐A), induces neuronal differentiation against neuroblastoma cells morphologically and also functionally. The differentiation and cytostatic effect induced by LB‐A was specific to neuroblastoma, Neuro 2A cells. To identify the target protein for LB‐A, a radioactive photoaffinity probe, [ 125 I]18‐(2′‐azido‐5′‐iodo‐benzoyloxy)‐LB‐18 ([ 125 I]azido‐LB‐18), was synthesized. As a result of in situ labeling experiments against Neuro 2A cells, a protein of M r 30 kDa was photolabeled specifically. This labeling was inhibited in the presence of LB‐A or the active analogs of LB‐A, whereas the inactive analogs showed no inhibitory effect on this labeling. These results suggest that this protein of M r 30 kDa is the target protein for LB‐A and may play an important role for the neuronal differentiation in neuroblastoma, Neuro 2A cells.