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An archaeal homing endonuclease I‐ Pog I cleaves at the insertion site of the neighboring intron, which has no nested open reading frame 1
Author(s) -
Nakayama Hitoshi,
Morinaga Yayoi,
Nomura Norimichi,
Nunoura Takuro,
Sako Yoshihiko,
Uchida Aritsune
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00497-6
Subject(s) - homing endonuclease , intron , open reading frame , intein , homing (biology) , dna , cleavage (geology) , endonuclease , biology , microbiology and biotechnology , gene , genetics , rna splicing , rna , peptide sequence , ecology , paleontology , fracture (geology)
Homing endonucleases (HEs) of the LAGLIDADG family cleave intron/inteinless cognate DNA at, or near, the insertion site (IS) of their own intron/intein. Here, we describe a notable exception to this rule. Two introns, Pog.S1205 (length 32 bp) and Pog.S1213 (664 bp), whose ISs are 8 bp apart, exist within the 16S rRNA gene of the archaeon Pyrobaculum oguniense . Pog.S1213 harbors a nested open reading frame (ORF) encoding a 22 kDa monomeric protein, I‐ Pog I, which contains two LAGLIDADG motifs and has optimal DNA cleavage activity at 90°C. Intriguingly, I‐ Pog I cleaves the Pog.S1205‐less substrate DNA in the presence or absence of Pog.S1213. The cleavage site (CS) of I‐ Pog I does not coincide with the IS of Pog.S1213 but with that of Pog.S1205. Thus, I‐ Pog I activity both promotes the homing of its own intron, Pog.S1213, and guarantees co‐conversion of the ORF‐less intron Pog.S1205.