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A pentatricopeptide repeat‐containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic male‐sterile rice
Author(s) -
Kazama Tomohiko,
Toriyama Kinya
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00480-0
Subject(s) - pentatricopeptide repeat , genetics , cytoplasmic male sterility , gene , biology , open reading frame , rna , microbiology and biotechnology , rna editing , peptide sequence
A fertility restorer gene ( Rf‐1 ) of [ ms‐bo ] cytoplasmic male sterility (CMS) in rice has been reported to be responsible for the processing of RNA of aberrant atp6 of mitochondria. We have carried out map‐based cloning of the Rf‐1 gene and found that a 4.7‐kb genomic fragment of a restorer line promoted the processing of aberrant atp6 RNA when introduced into a CMS line. The genomic fragment contained a single open reading frame encoding 18 repeats of the 35 amino acid pentatricopeptide repeat (PPR) motif. The cloned PPR gene is a possible candidate of Rf‐1 . A non‐restoring genotype was identified to have deletions within the coding region.