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Growth phase‐dependent changes in the subcellular localization of pre‐B‐cell colony‐enhancing factor 1
Author(s) -
Kitani Takako,
Okuno Sachiko,
Fujisawa Hitoshi
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00476-9
Subject(s) - cytoplasm , nucleus , 3t3 cells , subcellular localization , complementary dna , cdna library , microbiology and biotechnology , intracellular , biology , cell , cytokine , growth factor , cell culture , biochemistry , gene , transfection , immunology , genetics , receptor
A cDNA encoding the homolog of the human pre‐B‐cell colony‐enhancing factor (PBEF), a cytokine‐like secreted protein, was isolated from a rat cDNA library. This protein existed in both the cytoplasm and nucleus of the cells, and the amount was higher in the cytoplasm than in the nucleus of proliferating PC‐12 and Swiss 3T3 cells but higher in the nucleus than in the cytoplasm of the PC‐12 cells treated with nerve growth factor and the 3T3 cells grown to a confluent state. Thus, the so‐called PBEF is not a cytokine‐like secreted protein but an intracellular protein associated with the cell cycle.