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Identification of cell surface determinants in Candida albicans reveals Tsa1p, a protein differentially localized in the cell
Author(s) -
Urban C,
Sohn K,
Lottspeich F,
Brunner H,
Rupp S
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00455-1
Subject(s) - candida albicans , cell , heat shock protein , cell membrane , biotin , biochemistry , biology , microbiology and biotechnology , cell wall , enzyme , membrane protein , proteomics , chemistry , membrane , gene
To identify cell surface proteins of Candida albicans , the predominant fungal pathogen in humans, we have established an approach using a membrane impermeable biotin derivative in combination with affinity purification. We were able to identify 29 different proteins under two distinct conditions. Among mannoproteins, heat shock proteins and glycolytic enzymes we found thiol‐specific antioxidant‐like protein 1 (Tsa1p) to be differentially localized depending on the conditions applied. Only in hyphally grown cells Tsa1p was localized to the cell surface whereas in blastospores no surface but mainly nuclear localization was found. This indicates that cell surface expression of at least some proteins is mediated by differential translocation.