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Characterization of thioredoxin y , a new type of thioredoxin identified in the genome of Chlamydomonas reinhardtii
Author(s) -
Lemaire S.D.,
Collin V.,
Keryer E.,
Quesada A.,
Miginiac-Maslow M.
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00416-2
Subject(s) - chlamydomonas reinhardtii , thioredoxin , genome , biology , gene , chlamydomonas , arabidopsis , recombinant dna , genetics , biochemistry , mutant
The sequencing of the Arabidopsis genome revealed a multiplicity of thioredoxins (TRX), ubiquitous protein disulfide oxido‐reductases. We have analyzed the TRX family in the genome of the unicellular green alga Chlamydomonas reinhardtii and identified eight different thioredoxins for which we have cloned and sequenced the corresponding cDNAs. One of these TRXs represents a new type that we named TRX y . This most probably chloroplastic TRX is highly conserved in photosynthetic organisms. The biochemical characterization of the recombinant protein shows that it exhibits a thermal stability profile and specificity toward target enzymes completely different from those of TRXs characterized so far.