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pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferase
Author(s) -
Fraga Hugo,
Esteves da Silva Joaquim C.G.,
Fontes Rui
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00382-x
Subject(s) - polyphosphate , luciferase , adenylate kinase , oxidizing agent , chemistry , catalysis , combinatorial chemistry , biochemistry , phosphate , enzyme , organic chemistry , transfection , gene
Previous results have shown that an oxidizing product of firefly luciferin, dehydroluciferyl‐adenylate, is the main intermediate in the process of synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase (EC 1.13.12.7). However, we have found that the pH effects on the luciferase oxidizing processes and on the synthesis of dinucleoside polyphosphate are opposite: acidic assay media enhance the synthesis of dinucleoside polyphosphate and inhibit the oxidizing processes. The reason for this apparent contradiction lies on the activation effect of low pH on the adenylate transfer reaction from dehydroluciferyl‐adenylate to the acceptor nucleotide.