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CO 2 response for expression of ribulose‐1,5‐bisphosphate carboxylase/oxygenase genes is inhibited by AT‐rich decoy in the cyanobacterium
Author(s) -
Onizuka Takuo,
Akiyama Hideo,
Endo Sumiyo,
Kanai Shozo,
Hirano Masahiko,
Tanaka Satoshi,
Miyasaka Hitoshi
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00336-3
Subject(s) - decoy , oligonucleotide , oxygenase , transcription (linguistics) , dna , microbiology and biotechnology , transcription factor , biochemistry , biology , gene , binding site , chemistry , receptor , linguistics , philosophy
The CO 2 ‐regulatory function of the AT‐rich element in the promoter for ribulose‐1,5‐bisphosphate carboxylase/oxygenase ( rbc ) genes in the cyanobacterium Synechococcus sp. PCC7002 was analyzed using the transcription factor decoy approach. Double‐stranded phosphorothioate AT‐rich oligonucleotides with high affinity for a sequence‐specific DNA‐binding protein were successfully introduced into cyanobacterial cells in culture without any transfection reagent. The AT‐rich decoy oligonucleotides interfered with CO 2 regulation of rbc expression by blocking the binding of the sequence‐specific DNA‐binding protein, indicating that the AT‐rich element plays a critical role in CO 2 regulation for rbc genes. The decoy oligonucleotide approach to cyanobacteria provides a simple and excellent tool for investigating transcriptional regulation in vivo.