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DNA target sequence and FNR‐dependent gene expression
Author(s) -
Scott Colin,
Partridge Jonathan D,
Stephenson James R,
Green Jeffrey
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00312-0
Subject(s) - transcription (linguistics) , biology , genetics , gene , promoter , dna , escherichia coli , sequence (biology) , transcription factor , genome , microbiology and biotechnology , gene expression , philosophy , linguistics
FNR proteins are global transcription regulators that respond to fluctuations in environmental oxygen. They recognise a DNA target consisting of an inverted repeat, TTGATN 1 N 2 N 3 N 4 ATCAA (where N 1–4 represents a non‐conserved tetrad, NCT). Analysis of 68 known and predicted FNR sites from the Escherichia coli K12 genome revealed a bias toward A or T at positions N 2 and N 3 of the NCT. The effect of the NCT sequence on FNR‐dependent transcription in vivo was assessed using a series of class II and class I model promoters with different NCT sequences. Changing the NCT sequence did not affect basal activity but altered anaerobic induction by as much as an order of magnitude. Thus, the NCT sequence is a fundamental component in setting the dynamic range of the FNR switch.