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C/EBP homologous protein (CHOP) up‐regulates IL‐6 transcription by trapping negative regulating NF‐IL6 isoform
Author(s) -
Hattori Takayuki,
Ohoka Nobumichi,
Hayashi Hidetoshi,
Onozaki Kikuo
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00283-7
Subject(s) - ccaat enhancer binding proteins , gene isoform , transcription factor , chop , enhancer , microbiology and biotechnology , promoter , transcription (linguistics) , activator (genetics) , biology , nuclear protein , chemistry , gene expression , gene , endoplasmic reticulum , biochemistry , linguistics , philosophy
Interleukin‐6 (IL‐6) production is up‐regulated by several stimuli through the activation of transcription factors. We have previously demonstrated that CCAAT/enhancer binding protein homologous protein (CHOP) positively regulates IL‐6 production at the transcriptional level in the human melanoma cell line A375. In this study, we provide evidence that CHOP up‐regulates the IL‐6 transcription without binding to the IL‐6 promoter. CHOP dimerized more preferentially with an inhibitory isoform of nuclear factor for IL‐6 expression (LIP (liver‐enriched inhibitory protein)) than with a positively acting isoform (LAP, liver‐enriched activator protein). These results indicate that CHOP plays an important role in IL‐6 production without binding to its promoter, probably by trapping protein(s) such as LIP, which would otherwise inhibit IL‐6 transcription.