Premium
Disabled‐2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells
Author(s) -
Tseng Ching-Ping,
Huang Chien-Ling,
Huang Ching-Hui,
Cheng Ju-Chien,
Stern Arnold,
Tseng Chin-Hsiao,
Chiu Daniel Tsun-Yee
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00281-3
Subject(s) - k562 cells , small interfering rna , microbiology and biotechnology , mapk/erk pathway , transfection , biology , kinase , cellular differentiation , protein kinase a , chemistry , cell , cell culture , biochemistry , gene , genetics
Previous studies have shown that Disabled‐2 (DAB2) is up‐regulated during megakaryocytic differentiation of human K562 cells. To delineate the consequences of DAB2 induction, a DNA vector‐based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell–cell adhesion and mitogen‐activated protein kinase (MAPK) phosphorylation. The morphological changes and β3 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129–135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression.