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Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells
Author(s) -
Dever Gary,
Stewart Laura-Jayne,
Pitt Andrew R,
Spickett Corinne M
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00271-0
Subject(s) - phosphatidylcholine , phospholipid , chemistry , biochemistry , membrane
Chlorohydrins of stearoyl‐oleoyl phosphatidylcholine (SOPC), stearoyl‐linoleoyl phosphatidylcholine, and stearoyl‐arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (HL60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10–100 μM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4‐hydroxy‐2‐nonenal, but greater than that of hexanal, trans ‐2‐nonenal, and autoxidised palmitoyl‐arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.