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PCR‐linked in vitro expression: a novel system for high‐throughput construction and screening of protein libraries
Author(s) -
Rungpragayphan Suang,
Nakano Hideo,
Yamane Tsuneo
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00251-5
Subject(s) - in vitro , high throughput screening , computational biology , computer science , protein expression , microbiology and biotechnology , chemistry , biology , bioinformatics , biochemistry , gene
A novel entirely in vitro strategy for generation and screening of a combinatorial protein library in an array format has been developed and is experimentally demonstrated. The strategy exploits virtues of PCR and in vitro coupled transcription/translation. Our new approach provides high‐throughput construction and screening of the in vitro protein library, and compatibility with various selection methods.