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Human T‐cell leukemia virus type 1 Tax protein stimulates the interferon‐responsive enhancer element via NF‐κB activity 1
Author(s) -
Shimizu Toshiki,
Kawakita Shigenari,
Li Qin-Hua,
Fukuhara Shirou,
Fujisawa Jun-ichi
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00200-x
Subject(s) - enhancer , microbiology and biotechnology , interferon , biology , leukemia , gene , fibroblast , gene expression , chemistry , virology , cell culture , biochemistry , genetics
Gene expression in Rat‐1 fibroblast cells transformed by Tax from human T‐cell leukemia virus type 1 was studied using the reverse transcriptase polymerase chain reaction differential display technique. The analysis revealed eight genes that were upregulated and one gene that was suppressed in Tax‐transformed cells. Interestingly, at least four of the upregulated genes were interferon‐stimulated genes. Promoter analysis of the 2′,5′‐oligoadenylate synthetase gene, which was activated in both Tax‐transformed Rat‐1 cells and primary adult T‐cell leukemia cells, demonstrated that Tax indirectly activates its interferon‐responsive enhancer element in a nuclear factor‐κB pathway‐dependent manner, indicating a close association of interferon signaling with the transformation by Tax.